TIMP2 Antibody

SKU: orb606628

Description

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2). It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP9 or gelatinase B). TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: TIMP2/2044
• Immunogen: A portion of amino acids 48-220 from the human protein was used as the immunogen for this TIMP2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human placenta with TIMP2 antibody (clone TIMP2/2044). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using TIMP2 antibody (clone TIMP2/2044). These results demonstrate the foremost specificity of the TIMP2/2044 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free TIMP2 antibody (clone TIMP2/2044) as confirmation of integrity and purity.

Quick Database Links

UniProt

P16035