TNF alpha Recombinant Protein (HEK293 Expressed)

SKU: orb1227854

Description

TNF alpha Recombinant Protein (HEK293 Expressed)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: WB
• Application Notes:
  This recombinant protein can be used for WB. For research use only.

Key Properties

• Source: HEK293 cells
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha at 5 ug/mL (100 ul/well) can bind Humira with a linear range of 0.04-0.6 ng/mL. The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D.
• Target: TNF
• Tag: Tag free
• MW: 17.4 kDa
• Purity: >95% as determined by SDS-PAGE.
• Protein Sequence: Val 77 - Leu 233

Storage & Handling

• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Quick Database Links

Gene Symbol

TNF

UniProt

P01375

RefSeq:P01375