Prealbumin Antibody / Transthyretin / TTR

SKU: orb606853

Description

Prealbumin, also designated transthyretin, is a major thyroid-hormone binding protein involved in transporting thyroxine from the bloodstream to the brain. Prealbumin is located in the cytoplasm and in the vesicles of developing rat brain cells, and is thought to be transported there from the cerebrospinal fluid via endocytosis. Sequence variants of this protein have been identified in amyloid fibrils from patients with familial amyloidotic polyneuropathy (FAP), the most common form of hereditary systemic amyloidosis. Although the biologically active form of Prealbumin is a tetramer, the amyloidogenic intermediate is thought to be a monomeric species. Prealbumin also binds to the retinol carrier protein RBP (retinol-binding protein).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: order BSA/sodium azide-free format for coating,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Prealbumin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CPTC-TTR-1
• Immunogen: Recombinant human protein was used as the immunogen for this Prealbumin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Prealbumin antibody. These results demonstrate the foremost specificity of the CPTC-TTR-1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of human liver lysate with Prealbumin antibody (clone CPTC-TTR-1). Predicted molecular weight ~16 kDa.

Western blot testing of human kidney lysate with Prealbumin antibody (clone CPTC-TTR-1). Predicted molecular weight ~16 kDa.

SDS-PAGE analysis of purified, BSA-free Prealbumin antibody (clone CPTC-TTR-1) as confirmation of integrity and purity.

Quick Database Links

UniProt

P02766