VEGF Antibody / VEGFA

SKU: orb749841

Description

This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Research Area

Cardiovascular Research; Cell Biology

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 4-8ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the VEGF antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: VEGF/1063
• Immunogen: Human recombinant protein was used as the immunogen for the VEGF antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with VEGF antibody (clone VEGF/1063).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with VEGF antibody (clone VEGF/1063).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with VEGF antibody (clone VEGF/1063).

SDS-PAGE Analysis of Purified, BSA-Free VEGF Antibody (clone VEGF/1063). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P15692