VHH Antibody

SKU: orb420272

Description

VhH antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
• Reactivity: Camelus
• Application Notes:
  Anti-VhH antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein.
• Purity: Anti-VHH is directed against the llama VHH protein fragment. The product was purified from monospecific antiserum by protein A chromatography. Anti-VHH antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.08 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-VHH Antibody, single-domain antibody (sdAb), camelid VHH antibody

Western Blot of Rabbit anti-VHH antibody. Lane 1: Ladder (Opal Prestained). Lane 2: VHH protein. Lane 3: Llama IgG1 protein (p/n orb343832). Lane 4: Llama IgG2 protein (p/n orb343834). Lane 5: Llama IgG3 protein (p/n orb343833). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP (orb347654) rabbit secondary antibody at 1:70000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.

Western Blot of Rabbit anti-VHH antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: Llama IgG1 protein (p/n orb343832). Lane 3: Llama IgG2 protein (p/n orb343834). Lane 4: Llama IgG3 protein (p/n orb343833). Lane 5: VhH protein. Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:40000 for 45 min at RT. Block: BlockOut (p/n orb348637) overnight at 4°C. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.