VHH Antibody

SKU: orb750528

Description

VhH

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
• Reactivity: Camelus
• Application Notes:
  Anti-VhH antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein.
• Purity: Anti-VHH is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. Anti-VHH antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 90mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-VHH Antibody, single-domain antibody (sdAb), camelid VHH antibody

Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 µg lysate with 25 ng VhH. Primary antibody: Rabbit anti-VhH Antibody at 1:1000 o/n at 4°C. Secondary antibody: HRP Gt-a-Rb IgG (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VhH.

Western Blot Results of Rabbit Anti-VhH Antibody. Lane 1: Opal Prestained Molecular Weight. Lane 2: Llama IgG1 isotype control (p/n orb343832) reduced [0.05 µg]. Lane 3: Llama IgG2 isotype control (p/n orb343834) reduced [0.05 µg]. Lane 4: Llama IgG3 isotype control (p/n orb343833) reduced [0.05 µg]. Lane 5: VhH Control Protein reduced [0.05 µg]. Primary Antibody: Anti-VhH [Rabbit] Antibody at 1:1000 overnight at 2-8°C. Secondary Antibody: Anti-Rabbit IgG [Goat] Peroxidase Conjugated (p/n orb347673) at 1:40000 for 30 mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 1 hr at RT. Expected: recognizes the VhH protein ~15 kDa and detects the llama IgG1, IgG2 and IgG3 proteins. Exposure: 6.8 sec.