EIF4G2 Rabbit Polyclonal Antibody

SKU: orb213886

Description

The EIF4G2 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EIF4G2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Reactivity: Bovine, Human, Monkey, Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EIF4G2. The exact sequence is proprietary.
• Target: EIF4G2
• Molecular Weight: 102 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DAP5; Eukaryotic translation initiation factor 4 gamma 2; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; Death-associated protein 5; DAP-5; p97

Immunofluorescent analysis of EIF4G2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of mouse heart (1), rat heart (2) using EIF4G2 Rabbit Polyclonal Antibody orb213886 with dilution at 1:1000.

Immunohistochemical analysis of paraffin-embedded human liver cancer using EIF4G2 Rabbit Polyclonal Antibody orb213886 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse spleen using EIF4G2 Rabbit Polyclonal Antibody orb213886 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat stomach using EIF4G2 Rabbit Polyclonal Antibody orb213886 with dilution at 1:100.

Quick Database Links

Gene Symbol

EIF4G2

UniProt

P78344, Q62448

NCBI Gene ID

13690, 1982