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Description
Research Area
Images & Validation
−| Tested Applications | IHC |
|---|---|
| Dilution Range | Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat |
| Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TGF beta 1/TGFB1, identical to the related mouse and rat sequences. |
| Target | Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide |
| Molecular Weight | 120 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody. TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-TGF Beta 1/TGFB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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TGF beta 1/TGFB1 Rabbit Polyclonal Antibody (orb1098055)
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