CX43 Antibody

SKU: orb11602

Description

Goat polyclonal to connexin 43, which is a member of the connexin family. Cx43 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. This protein is the major protein of gap junctions in embryonic development and in the heart that are thought to have a crucial role in the synchronized contraction of the heart.

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:250-1:800, IHC-F:1:250-1:800
• Reactivity: Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E. coli. Antigen Sequence: VKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI
• Target: Gap junction protein, alpha 1, 43kDa
• Purification: Epitope affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Polyclonal antibody supplied as a 200 or 500 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum and it is identical to AB1600.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AVSD3, CX43, DFNB38, gap junction protein, alpha 1, 43kDa, GJA1, GJAL HSS, HLHS1, ODDD antibody.

Western blot analysis of COS-7 cell lysate using Connexin 43 antibody

Confocal immunofluorescence analysis of primary RPE cells using Connexin 43 antibody

Quick Database Links

Gene Symbol

Gap junction protein, alpha 1, 43kDa

Ahmad Mostaar 1, Zahra Behroozi 2, Ali MotamedNezhad 3, Sourosh Taherkhani 4, Negin Mojarad 5, Fatemeh Ramezani 6, Atousa Janzadeh 7, Pooya Hajimirzaie The effect of intra spinal administration of cerium oxide nanoparticles on central pain mechanism: An experimental study J Bioenerg Biomembr, (2024)

PubMed: 39102102

Applications

WB

Reactivity

Rat