Recombinant CCNE1 Antibody / Cyclin E1

SKU: orb1151663

Description

Rabbit monoclonal antibody to Cyclin E1

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant CCNE1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Clone No.: CCNE1/4935R
• Immunogen: A portion of amino acids 10-176 was used as the immunogen for the recombinant CCNE1 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human HCT-116 cell lysate using recombinant CCNE1 antibody (clone CCNE1/4935R). Predicted molecular weight ~47 kDa.

IHC staining of FFPE human endometrium with recombinant CCNE1 antibody (clone CCNE1/4935R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian carcinoma tissue with recombinant CCNE1 antibody (clone CCNE1/4935R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CCNE1 antibody (clone CCNE1/4935R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P24864