Recombinant Cyclin E1 Antibody / CCNE1

SKU: orb1151689

Description

Mouse monoclonal antibody to Cyclin E1

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Cyclin E1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rCCNE1/4936
• Immunogen: A portion of amino acids 10-176 was used as the immunogen for the Cyclin E1 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human HCT116 cell lysate using recombinant Cyclin E1 antibody (clone rCCNE1/4936). Predicted molecular weight ~47 kDa.

IHC staining of FFPE human ovarian carcinoma tissue with recombinant Cyclin E antibody (clone rCCNE1/4936). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue with recombinant Cyclin E1 antibody (clone rCCNE1/4936). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Cyclin E1 antibody (clone rCCNE1/4936) as confirmation of integrity and purity.

Quick Database Links

UniProt

P24864