Slc2a2 Antibody

SKU: orb420320

Description

GLUT2 antibody

Images & Validation

• Tested Applications: ELISA, FLISA, IF, IHC, WB
• Dilution Range: ELISA: 1:30,000-1:90:000, FLISA: 1, IHC: 4µg/mL, WB: 1:500-1:1000
• Reactivity: Human, Mouse
• Application Notes:
  Anti-Glut-2 antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57.1 kDa in size corresponding to Glut2 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Glut2 protein.
• Target: Slc2a2
• Purity: This affinity-purified Glut-2 antibody is directed against Glut2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with Glut2 from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Glut2 antibody, Solute carrier family 2 facilitated glucose transporter member 2, Glucose transporter type 2 liver, GLUT-2 antibody, Slc2a2, Glut 2 Antibody

Immunohistochemistry of Anti-Glut2 Antibody. Tissue: mouse kidney. Antigen retrieval: Heat Induced, slides incubated in sodium citrate buffer for 1hr at 90°C. Primary: Rabbit Anti-Glut2 Antibody at 5 µg/ml overnight. Blocking: 2% goat serum in TBST. Secondary: Alexa 488 at 1 µg/ml for 2hrs at room temperature.

Immunohistochemistry with anti-Glut2 antibody showing Glut2 staining in nucleus and cytoplasm of ductal epithelium and of renal glomeruli in human kidney at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH6.2 and then incubated with rabbit anti-mouse Glut2 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).

Western Blot of Rabbit anti-Glut2 antibody. Lane 1: HEK293 lysate (p/n orb348669). Load: 10 µg per lane. Primary antibody: Glut2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: Blocking Buffer (p/n orb348637) overnight at 4°C. Predicted/Observed size: ~60 kDa.

Western Blot of Rabbit Anti-Glut2 Antibody. Lane 1: Molecular Weight. Lane 2: Mouse Kidney WCL (p/n orb348716). Lane 3: MEF WCL (p/n orb348722). Lane 4: 3T3 WCL (p/n orb348714). Lane 5: HeLa WCL (p/n orb348668). Lane 6: HEK293 WCL (p/n orb348669). Load: 20 µg per pane. Primary antibody: Anti-Glut2 at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) 1:40000 diluted with orb348644 for 30 minutes at RT. Blocked with (p/n orb348644) Buffer. Expect: ~57.1 kDa. Exposure: 30 sec.

Quick Database Links

Gene Symbol

Slc2a2

UniProt

P14246

RefSeq (Protein):NP_112474.2