Slc2a2 Antibody

SKU: orb420321

Description

GLUT2 antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:30,000-1:90:000, IHC: 4µg/mL, WB: 1:500-1:1000
• Reactivity: Human, Mouse
• Application Notes:
  Anti-Glut-2 antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57.1 kDa in size corresponding to Glut2 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Glut2 protein.
• Target: Slc2a2
• Purity: This affinity-purified Glut-2 antibody is directed against Glut2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with Glut2 from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Glut2 antibody, Solute carrier family 2 facilitated glucose transporter member 2, Glucose transporter type 2 liver, GLUT-2 antibody, Slc2a2, Glut 2 Antibody

Immunohistochemistry of Anti-Glut2 Antibody. Tissue: mouse kidney. Antigen retrieval: Heat Induced, slides incubated in sodium citrate buffer for 1hr at 90°C. Primary: Rabbit Anti-Glut2 Antibody at 5 µg/ml overnight. Blocking: 2% goat serum in TBST. Secondary: Alexa 488 at 1 µg/ml for 2hrs at room temperature.

Immunohistochemistry with anti-Glut2 antibody showing Glut2 staining in nucleus and cytoplasm of ductal epithelium and of renal glomeruli in human kidney at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH6.2 and then incubated with rabbit anti-mouse Glut2 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).

Western Blot of Rabbit anti-Glut2 antibody. Lane 1: HEK293 lysate (p/n orb348669). Load: 10 µg per lane. Primary antibody: Glut2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: Blocking Buffer (p/n orb348637) overnight at 4°C. Predicted/Observed size: ~60 kDa.

Western Blot of Rabbit Anti-Glut2 Antibody. Lane 1: Molecular Weight. Lane 2: Mouse Kidney WCL (p/n orb348716). Lane 3: MEF WCL (p/n orb348722). Lane 4: 3T3 WCL (p/n orb348714). Lane 5: HeLa WCL (p/n orb348668). Lane 6: HEK293 WCL (p/n orb348669). Load: 20 µg per pane. Primary antibody: Anti-Glut2 at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) 1:40000 diluted with orb348644 for 30 minutes at RT. Blocked with (p/n orb348644) Buffer. Expect: ~57.1 kDa. Exposure: 30 sec.

Quick Database Links

Gene Symbol

Slc2a2

UniProt

P14246

RefSeq (Protein):NP_112474.2