EBF1/COE1 Antibody

SKU: orb20040

Description

Goat polyclonal antibody to EBF1

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3-0.1 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1 μg/ml.Experiment Notes: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.

Key Properties

• Clonality: Polyclonal
• Target: EBF1 / COE1
• Protein Sequence: HNQLPALANTSV
• Molecular Weight: 64.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EBF1 antibody, anti early B-cell factor 1 antibody, anti COE1 antibody, anti EBF antibody, anti O/E-1 antibody, anti OLF1 antibody, anti Collier, Olf and EBF transcription factor 1 antibody, anti early B-cell factor olfactory neuronal transcription factor 1 antibody

orb20040 (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

EBF1 / COE1

NCBI Gene ID

1879