STAT3 Antibody

SKU: orb19306

Description

Goat polyclonal antibody to STAT3

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 75kDa band observed in Human Heart and Human Liver lysates (calculated MW of 83.1kDa according to NP_998827.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: STAT3
• Protein Sequence: CRPESQEHPEADP
• Molecular Weight: 88.1; 88.0; 83.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti STAT3 antibody, anti signal transducer and activator of transcription 3 (acute-phase response factor) antibody, anti APRF antibody, anti FLJ20882 antibody, anti MGC16063 antibody, anti DNA-binding protein APRF antibody, anti acute-phase response factor antibody, anti signal transducer and activator of transcription 3 antibody

orb19306 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Human Heart lysate using STAT3 antibody

Immunohistochemical staining of Human Kidney using STAT3 antibody

Quick Database Links

Gene Symbol

STAT3

NCBI Gene ID

6774