IGFBP7 Antibody

SKU: orb1272658

Description

IGFBP7 Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Human IGF-BP3 specific antibody was purified by affinity chromatography employing immobilized hIGF-BP3 matrix.
• Target: IGFBP7
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AGM, PSF, TAF, FSTL2, IBP-7, MAC25, IGFBP-7, RAMSVPS, IGFBP-7v, IGFBPRP1, Insulin-like growth factor-binding protein 7, IGFBP-rP1

To detect hIGF-BP7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP7 (orb1272740) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7.

To detect hIGF-BP7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hIGF-BP7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained HeLa and U-251 MG cells. The primary antibody was incubated at 2.0 mg/ml overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

IGFBP7

UniProt

Q16270

RefSeq:Q16270