CHUK Antibody

SKU: orb1239457

Description

CHUK Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  IKK alpha can be used for detection of IKK alpha by Western blot at 1 μg/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: IKK alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human IKK alpha.The immunogen is located within the last 50 amino acids of IKK alpha.
• Target: CHUK
• Molecular Weight: 85 kDa
• Purification: IKK alpha Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha

Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution.

Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1 µg/mL.

Immunofluorescence of IKK alpha in Jurkat cells with IKK alpha antibody at 10 µg/mL.

Immunofluorescence of IKKa in Jurkat cells with IKK alpha antibody at 20 µg/mL. Green: IKK alpha Antibody (orb1239457) Blue: DAPI staining.

Immunocytochemistry of IKKa in Jurkat cells with IKK alpha antibody at 2 µg/mL.

Quick Database Links

Gene Symbol

CHUK

UniProt

O15111

RefSeq:AF009225