LDHA Antibody

SKU: orb344339

Description

Lactate Dehydrogenase antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IF: 1:200, IP: 1:100, WB: 1:1,000 - 1:5,000
• Reactivity: Human, Rabbit
• Application Notes:
  LACTATE DEHYDROGENASE antibody is suitable for use in ELISA, western blot, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
• Target: LDHA
• Purity: Anti-LACTATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase Biotin Conjugated antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.

Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.

Quick Database Links

Gene Symbol

LDHA

UniProt

P13491

RefSeq:126050