S100B Antibody

SKU: orb1261803

Description

S100B Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100B (NP_006263.1).
• Target: S100B
• Molecular Weight: Observed: 11kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

Western blot analysis of extracts of various cell lines, using S100B antibody at 1:1000 dilution.

Immunofluorescence analysis of U-251MG cells using S100B antibody at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse brain using S100B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain using S100B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

S100B

UniProt

P04271