ZEB2 Antibody

SKU: orb1238535

Description

ZEB2 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ZEB2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZEB2.The immunogen is located within the last 50 amino acids of ZEB2.
• Target: ZEB2
• Molecular Weight: Predicted: 134 kDa Observed: 135 kDa
• Purification: ZEB2 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ZEB2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ZEB2 Antibody: SIP1, SIP-1, ZFHX1B, HSPC082, SMADIP1, KIAA0569, SIP1, ZFX1B, HRIHFB2411, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1

Western blot analysis of ZEB2 in EL4 cell lysate with ZEB2 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/mL.

Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/ml. Red: ZEB2 Antibody (orb1238535) Blue: DAPI staining.

Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

ZEB2

UniProt

O60315

RefSeq:AAI27103